bio rad tesee sap rapid test  (Bio-Rad)

Journal: EFSA Journal

Article Title: The European Union summary report on surveillance for the presence of transmissible spongiform encephalopathies ( TSE ) in 2022

doi: 10.2903/j.efsa.2023.8384

Figure Lengend Snippet: Description of the CWD cases in 2022.

Article Snippet: NO , 1 , Wild deer , European moose (as animal) , Female , ≥12 months , FC , Obex (as part‐nature) , Screening , Bio‐Rad TeSeE SAP rapid test (using the CWD protocol) , POS.

Techniques: Western Blot

Journal: Veterinary research

Article Title: Experimental transmission of ovine atypical scrapie to cattle.

doi: 10.1186/s13567-023-01224-3

Figure Lengend Snippet: Figure 2 Western immunoblot examination of the brainstem of TSE suspect 06/17. Atypical scrapie sheep 76/06 was the inoculum donor of test group 1, which included clinical suspect 6/17. PrPres was not detectable with any of the antibodies in the samples from steer 6/17.

Article Snippet: Fresh obex tissue was used to detect proteinase-resistant prion protein (PrPres) by rapid TSE test, BioRad TeSeE ELISA (BioRad Laboratories), carried out according to the manufacturer’s instructions and WB according to previously published protocols using monoclonal antibodies Sha31 (BioRad Laboratories, included in the WB kit, dilution as per WB kit protocol), which targets the epitope 156YEDRYYRE163, 12B2 (Central Veterinary Institute of Wageningen UR; dilution 0.5 μg/mL), which targets epitope 101WGQGG105 and SAF84 (Cayman Chemicals; dilution 0.4 μg/mL), which targets epitope 175RPVDQY180 of the bovine PrP [30].

Techniques: Western Blot